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Image Search Results
Journal: Synapse (New York, N.y.)
Article Title: Harnessing Miniscope Imaging in Freely Moving Animals to Unveil Migraine Pathophysiology and Validate Novel Therapeutic Strategies
doi: 10.1002/syn.70001
Figure Lengend Snippet: Miniscope imaging enables monitoring of vascular dynamic in a freely moving animal model of migraine. (A) A standardized workflow for vascular imaging in a migraine model using miniscopes. Created in BioRender. Ye, T. (2024) BioRender.com/p37×969. (B) A newly developed algorithm enables accurate diameter estimation of a broad range of vessel sizes. First, users manually draw a line that bisects a vessel to be analyzed (left, middle). To estimate the vessel diameter, the algorithm uses the pixel intensity profile along each label, calculating the local minimum on each side of the curve, and comparing to the global maximum. On each side of the curve, the pixel with the value just above the half point between the global maximum and the corresponding minimum is determined to be the edge of the vessel. The distance between the two pixels is calculated as the vessel diameter (right). (C) Comparison of algorithm diameter estimates versus manual estimates of the same vessels. Correlation between the two methods is high (Pearson R = 0.992). (D) Example images before (top left) and 30 min after levcromakalim administration (top right). Orange lines overlaying the images denote the original width of the underlying vessels. Teal lines show the change in vessel width after levcromakalim administration. Traces (below) show the algorithmic diameter estimation of the corresponding vessels in the images above, normalized to the predrug mean. Dashed gray lines indicate the timing of levcromakalim administration. (E) Synchronized behavior videos can be collected alongside miniscope acquisition. The nVision deep learning algorithm can automatically detect a mouse in the camera's field of view (top). Position data can be preserved frame by frame. Mouse position data from the first 5 min of a session is plotted below.
Article Snippet: From the behavioral video, animal position was estimated frame‐by‐frame using the
Techniques: Imaging, Animal Model, Comparison